Command Shortcut Description New N Creates a new image or stack Open O Opens a TIFF, GIF, JPEG, BMP, DICOM or FITS Open Samples Shift-B Opens "Blobs" example image Close W Closes the active window Save S Saves active image in Tiff format Revert R Revert to saved version of image P Print active image Undo Z Reverses the effect of the last operation Cut X Copy image to clipboard and clear selection Copy C Copy image to clipboard Paste V Paste clipboard selection into active image Clear backspace Erases selection to background color Select All A Select entire image Select None Shift-A Delete selection Restore Selection Shift-E Restores ROI Fill F Fills selection in foreground color Draw D Draw selection Invert Shift-I Invert image or selection Adjust Contrast Shift-C Adjust brightness and contrast Adjust Threshold Shift-T Adjust threshold levels Show Info I Displays information about active image Next Slice > Advance to next stack slice Previous Slice < Backup up to previous stack slice Start Animation = Starts/stops stack animation Duplicate Shift-D Duplicates active image or selection Scale E Scale image or selection Smooth Shift-S 3x3 unweighted smoothing Find Edges Shift-F Performs Sobel edge detection Repeat Command Shift-R Repeats previous command Measure M Displays statistics about active image or selection Histogram H Displays a histogram of the active window or selection Plot Profile K Displays density profile plot of current selection ImageJ enter Brings ImageJ window to front Put Behind tab Switches to next image window
Alt Key Modifications
Image/Adjust/Threshold: Adjusting Min also adjusts Max
Image/Stacks/Add Slice: Insert before current slice
Image/Stacks/Next Slice: Skip nine slices
Image/Stacks/Previous Slice: Skip nine slices
Image/Duplicate: Don't show dialog
Image/Colors: Alt-click to "pick up" background color
Process/Equalize: Do classic histogram equalization
Process/Subtract Background: Show background image
Analyze/Plot Profile: For rectangular selections, generate row average plots
Analyze/Tools/Analyze Line Graph: Show intermediate image
Plugins/Utilities/Monitor Memory: Simulate 640x480 frame grabber
Any User Plugin: Load using new class loader
Space Bar Modifications
Any Tool: Temporarily switch to the "hand" (scrolling) tool
Moving through a stack: Automatically adjust min/max display values
Function Keys
Use the function keys to switch tools. F1 selects the rectangle tool, F2 selects the oval tool, etc.Arrow Keys
Use the arrow keys to move selection outlines one pixel at a time. Resize rectangular and oval selections by holding down the the alt (option) and while using the arrow keys.